Abstract

Objective To observe the effects of ulinastatin (UTI) on malondialdehyde (MDA) and superoxide dismutase (SOD) in plasma and lung tissue in the rats with severe gut-origin sepsis.Methods One hundred and fourteen Wistar rats were randomly divided into four groups:normal group(n=6),sham control group(n=30),septic group(n=30),UTI3 h group(n=30) and UTII2 h group (n=18). Blood and lung samples were obtained for detecting MDA and SOD at 6,12,24,48,72 h after CLP. Gutorigin sepsis model was induced by the classic technique of cecal ligation and puncture (CLP). The administration of UTI was injected into abdominal cavity at 3 or 12h after the end of CLP and reinjected after 12 h interval. Blood samples was taken in the normal and experimental rats at 6,12,24,48,72 h after sham-operated or CLP for the determinations of MDA and SOD in plasma and lung tissue.Results In the septic group rats,MDA in plasma and lung tissue and lung W/D ratio were higher but SOD was lower significantly than that in the sham control group (P<0.05). As compared with SEP group,MDA in plasma and lung tissue and lung W/D ratio were decreased significantly in the UTI3 h and UTI12 h groups (P<0.05),and then still higher than that in sham control group. SOD in plasma and lung tissue were increased significantly in the UTI3 h and UTI12 h groups(P<0.05),and then still lower than that in sham control group. W/D in lung tissue was positively correlated with SOD in lung tissue or plasma (P<0.01),but negatively with MDA in lung tissue or plasma (P<0.01).Conclusion It is suggested that ulinastatin could improve oxyradicalmediated lung injury induced by gut-origin sepsis and block the vicious cycle of oxygen free radical cascades,and then relieve damage of remote organs. Key words: Ulinastatin; Gut-origin septic status; MDA; SOD

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