Ulinastatin relieves oxyradical-mediated lung injury induced by gut-origin septic status in rats

Abstract

Objective To observe the effects of ulinastatin (UTI) on malondialdehyde (MDA) and superoxide dismutase (SOD) in plasma and lung tissue in the rats with severe gut-origin sepsis.Methods One hundred and fourteen Wistar rats were randomly divided into four groups:normal group(n=6),sham control group(n=30),septic group(n=30),UTI3 h group(n=30) and UTII2 h group (n=18). Blood and lung samples were obtained for detecting MDA and SOD at 6,12,24,48,72 h after CLP. Gutorigin sepsis model was induced by the classic technique of cecal ligation and puncture (CLP). The administration of UTI was injected into abdominal cavity at 3 or 12h after the end of CLP and reinjected after 12 h interval. Blood samples was taken in the normal and experimental rats at 6,12,24,48,72 h after sham-operated or CLP for the determinations of MDA and SOD in plasma and lung tissue.Results In the septic group rats,MDA in plasma and lung tissue and lung W/D ratio were higher but SOD was lower significantly than that in the sham control group (P＜0.05). As compared with SEP group,MDA in plasma and lung tissue and lung W/D ratio were decreased significantly in the UTI3 h and UTI12 h groups (P＜0.05),and then still higher than that in sham control group. SOD in plasma and lung tissue were increased significantly in the UTI3 h and UTI12 h groups(P＜0.05),and then still lower than that in sham control group. W/D in lung tissue was positively correlated with SOD in lung tissue or plasma (P＜0.01),but negatively with MDA in lung tissue or plasma (P＜0.01).Conclusion It is suggested that ulinastatin could improve oxyradicalmediated lung injury induced by gut-origin sepsis and block the vicious cycle of oxygen free radical cascades,and then relieve damage of remote organs. Key words: Ulinastatin; Gut-origin septic status; MDA; SOD