Abstract
OBJECTIVE: Lansoprazole (Takepron; Takeda) is extensively used in the treatment of acid-related diseases. There are no prior studies of the effects of antisecretion drugs on parietal cells, but we recently developed a rabbit parietal cell model. This allowed us to compare the antisecretory effects of lansoprazole and cimetidine in vitro in the present study. METHODS: Parietal cells were isolated using cell elutriation and continuous density gradient centrifugation. The effect of cimetidine and lansoprazole on histamine-induced acid secretion in rabbit parietal cells was investigated by measuring the accumulation of 14C-aminopurine. RESULTS: The purity and viability of isolated parietal cells was >80 and 95%, respectively. Lansoprazole and cimetidine both significantly inhibited histamine-stimulated acid secretion (10−4 mol/L), but the 50% inhibition concentration (IC50) of the two treatments was quite different. The IC50 of cimetidine was 3.70 × 10−5 mol/L, and that of lansoprazole was 9.59 × 10−8 mol/L. CONCLUSIONS: Lansoprazole is much more effective than cimetidine in the in vitro inhibition of histamine-stimulated acid secretion in rabbit parietal cells. This study developed a model for the study of acid inhibition drugs.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
