Çukurova Bölgesi Pamuk Alanlarında Bemisia tabaci (Gennadius)’de Aşırı Düzeyde Sitokrom P450 CYP6CM1 Gen Ekspresyonu

Abstract

In Cukurova region, neonicotinoid group insecticides are widely used in cotton cultivation areas for controlling B. tabaci; as a result of this use, there is a problem of resistance in these areas. In this study, cytochrome P450 CYP6CM1 relative expression levels, which are the indicators of imidacloprid resistance, were analyzed. Five different B. tabaci populations were collected from Adana city cotton cultivation areas in 2018 and the indicators were observed. On the other hand, GST gene relative expression profile, which is an efficient factor in metabolic resistance, cytochrome P450, carboxylesterase (AcHE) and Glutathion S transferase (GST) enzyme activities were determined. The highest cyp6cm1 and GST genes relative activities were determined in Meletmez population; the activity level was 18, 2 and 9 times higher than susceptible population. On the other hand, the highest and lowest cytochrome P450 enzyme activity was observed in Meletmez and Solaklı populations, respectively. According to the analyses, it can be concluded that there is extensive imidacloprid and neonicotinoid resistance of B. tabaci populations in Çukurova Region cotton cultivation areas. As a result, excessive gene expression and high-level enzyme activities indicate that there is a resistance in the studied area based on enforced monooxygenases activity.