Abstract
Mercury (Hg) is a heavy metal could pollute the river. Hg impacts on aquatic organism chronically. The influence of chemical toxic on aquatic organisms could be determined using toxicity (lethal and sublethal) tests. Osteochilus hasselti could be an object for toxicity test. A research, aiming to find LC50-96h concentration, was to find letal and subletal effects of Hg on erythrocyte and hematocryte changes. An experimental method applied Completely Randomized Design. The research was devided into 3 stages, i.e. preliminary, lethal toxicity (LC50-96h) and sublethal toxicity tests, in triplicates. Sampling of sublethal test was performed after an exposure time of 96 h (4 days) and 288 h (12 days). Lethal toxicity test data were analyzed as probit and data from sublethal toxicity test were F-tested. The result showed that LC50-96h of Hg on Osteochilus hasselti was 0.39 mg/L. The sublethal effect of Hg decreased erythrocyte ad hematocryte counts in parallel with increasing its concentration and its exposure time.Keywords: Hg, toxicity, LC50-96 hours, erythrocytes, hematocrits.
Highlights
Merkuri (Hg) adalah unsur logam yang sangat penting dalam teknologi di abad modern saat ini (Hadi, 2013)
Osteochilus hasselti could be an object for toxicity test
The research was devided into 3 stages
Summary
Merkuri (Hg) adalah unsur logam yang sangat penting dalam teknologi di abad modern saat ini (Hadi, 2013). Logam berat merkuri masuk ke dalam jaringan tubuh melalui beberapa jalan, yaitu saluran pernapasan, pencernaan dan penetrasi melalui kulit. Masuknya logam melalui saluran pernapasan dengan cara ikan mengabsorbsi merkuri langsung dari air dengan melewati insang kemudian dibawa oleh darah untuk diedarkan ke seluruh tubuh. Dampak langsung polutan terhadap ikan bersifat lethal dan sublethal, dimana dapat menimbulkan efek genetik terhadap biota yang bersangkutan. Ikan yang dipapar merkuri dengan konsentrasi ≥0,16 ppm menunjukkan penurunan tingkat kelangsungan hidup dan laju pertumbuhan yang disebabkan oleh peningkatan stress dan kerusakan organ (Nirmala et al, 2012). Konsentrasi merkuri di dalam air ≥3 ppm dapat menyebabkan kematian masal pada suatau perairan (Tyas,2013). Penelitian tentang toksisitas letal 50% dalam waktu pemaparan 96 jam (LC5096 jam) dan subletal Hg terhadap spesies asli ikan nilem (Osteochilus hasselti ) perlu dilakukan
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