Abstract
ABSTRACTGuava is one of the plants that can traditionally be used for the treatment of diseases. Many kinds of guava, one of which is the Australian guava has the characteristics of roots, stems, leaves, dark red fruit. This study aims to determine the toxicity of the ethanol extract of Australian guava leaves (Psidium guajava L) using the BSLT (Brine Shrimp Lethality Test) method and determine the chemical content of Australian guava leaves (Psidium guajava L). The extract was made by the ultrasonic method using 96% ethanol solvent. Toxicity tests were carried out using 48-hour-old Artemia salina Leach shrimp larvae. The toxic effect of the extract was identified by the percentage of shrimp larvae mortality using probit analysis (LC50). From the research results, phytochemical content includes tannins, flavonoids, alkaloids, terpenoids and saponins, and flavonoid compounds have the highest content compared to the others. Research shows that the ethanol extract of Australian guava leaves is of a moderate category (LC50 441,977 ppm).Keywords :Australia guava leaves, BSLT, Artemia salina L, Ultrasonic
Highlights
Jambu biji merupakan salah satu tanaman yang secara tradisional dapat dimanfaatkan untuk pengobatan penyakit
One of which is the Australian guava has the characteristics of roots, stems, leaves, dark red fruit
This study aims to determine the toxicity of the ethanol extract of Australian guava leaves (Psidium guajava L) using the BSLT (Brine Shrimp Lethality Test) method and determine the chemical content of Australian guava leaves (Psidium guajava L)
Summary
Sebanyak 2 mL sampel ditambahkan dengan 3 tetes HCl pekat dan 1 tetes H2SO4 pekat. Jika terbentuk warna merah atau ungu maka positif mengandung terpenoid. 2. Identifikasi Tanin Sebanyak 0,1 gram ekstrak ditambahkan 10 mL aquades, disaring lalu filtratnya ditambahkan reagen besi (III) klorida (FeCl3) 1% 5 mL. Warna biru tua atau hitam menunjukkan adanya tannin
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