Abstract
Based on the results of previous studies conducted by researchers in January 2018 showed that the Total Plate Count (ALT) of Jamu Gendong in several villages in Talang sub-district did not meet the quality safety standards (ALT value of more than 104). The high ALT value indicates the presence of bacteria contained in the tamarind tamarind herbal medicine. One of the bacteria that often contaminates food and beverages or drugs is Escherichia coli. E. coli bacteria is a characteristic bacterium contaminated with food or drug samples. The purpose of this study is to test for the presence or absence of E coli bacteria in gedong kunyit asem herbal medicine in Tegal district. The identification test of E. coli bacteria was carried out through two techniques, namely staining and bacterial culture techniques. For coloring techniques, gram coloring tests will be carried out, while bacterial cultures include blood agar plates, and Macconkey agar media. The results showed that gram staining, as well as culture in the media of Blood Agar Plate and Macconkey Agar showed the presence of E. coli bacteria in samples of tamarind ancient tamarind in several villages in Tegal Regency Keywords: identification test, Escherichia coli bacteria, Jamu genndong, Kunyit asem.
Highlights
Zaman modern yang penuh dengan kemudahan hidup tak berbanding lurus dengan munculnya berbagai penyakit di muka bumi
The results showed that gram staining, as well as culture in the media of Blood Agar Plate and Macconkey Agar showed the presence of E. coli bacteria in samples of tamarind ancient tamarind in several villages in Tegal Regency
Jakarta: Erlangga. [7] I Made Nugraha Gunamanta Sabudi , Made Agus Hendrayana2. 2017. identifikasi bakteri Eschericia coli serotipe o157 dengan media sorbitol mac conkey agar (smac) pada buah semangka potong dari pedagang buah kaki lima di kota denpasar Volume 6 no
Summary
Zaman modern yang penuh dengan kemudahan hidup tak berbanding lurus dengan munculnya berbagai penyakit di muka bumi. Banyak pula masyarakat yang beralih menggunakan obat alam karena dipandang lebih aman dan tak beresiko efek samping. Dengan berbagai alasan sepeti lebih murah serta aman itulah yang membuat masyarakat pedesaan khususnya banyak mengkonsumsi obat herbal. Salah satu jamu yang masih dijajakan di beberapa desa di kabupaten Tegal yaitu jamu gendong. Berdasarkan hasil penelitian sebelumnya yang telah dilakukan peneliti pada bulan Januari 2018 menunjukkan bahwa Angka Lempeng Total (ALT) Jamu Gendong kunyit asem di beberapa desa di kecamatan Talang belum memenuhi standar keamanan mutu (nilai ALT lebih dari 104 )[3]. Keberadaan bakteri ini akan menyebabkan berbagai penyakit salah satunya yaitu diare. Berdasarkan hal tersebut, maka peneliti tertarik untuk mengidentifikasi keberadaan bakteri E. coli yang terkandung dalam jamu gendong kunyit asam di beberapa desa di kabupaten Tegal. Untuk teknik pewarnaan ada uji pewarnaan gram sedangkan kultur bakteri meliputi media agar darah (Blood Agar Plate), dan media agar Macconkey
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