Uji Efek Kelasi Ekstrak Etanol Daun Mangifera foetida pada Feritin Serum Penderita Talasemia di Rumah Sakit Cipto Mangunkusumo, Tahun 2012

Abstract

Treatment of thalassemia with blood transfusion causing iron accumulation in the organs and damaging cells. Chelating agent, deferoxamine causes side effects and expensive. Therefore, it’s needed a safer and cheaper treatment by utilizing natural ingredients which have chelating effect. Water extract of Mangifera foetida leaf has chelating effect on serum thalassemia patients, but there was no research the effects in the ethanol extract. The purpose of this study was to prove the effects of ethanol extract as a chelating agent. This study used an experimental study using seven serums of patients with thalassemia by ex vivo and devided into seven treatments: serum, mangiferin, mangiferin plus serum, etanol extract 0.5 mg and 0.75 mg, etanol extract 0.5 mg and 0.75 plus serum, however only four treatments will be analized: serum, mangiferin plus serum, etanol extract 0.5 mg and 0.75 mg plus serum. They were measured in a spectrophotometer with (λ)=280 nm. The result by One Way Anova statistical test showed that the ethanol extract of M. foetida leaf 0.5 mg and 0.75 mg has the chelating effect when it compared to negative control (p <0.001). Post hoc test showed that the ethanol extract 0.5 mg has the same chelating effect with ethanol extract 0,75 mg (p = 0.133). Ethanol extract 0.5 mg has the same effect of iron chelation with the mangiferin (p=0.52), while ethanol extract 0.75 mg has different effect (p=0.001). The differences of chelating effect maybe caused by the differences of extract dose.