UJI AKTIVITAS STIMULANSIA EKSTRAK DAUN BANGUN-BANGUN (Coleus amboinicus L.) DENGAN METODA ROTAROD

Abstract

The leaves of the Coleus amboinicus commonly consumed in North Sumatra, are known for their diverse benefits, including their use as a stimulant. These leaves contain various chemical compounds such as carvacrol, quercetin, and alkaloids. This study aims to determine the effectiveness and optimal dosage of the stimulant effects of the bangun-bangun leaf extract using the rotarod method. The extraction method used was maceration. The bangun-bangun leaf extract (EDB) was tested as a stimulant on 30 male mice, divided into six groups: negative control (CMC-na 5%), positive control (caffeine 0.36 mg), and treatment groups given different doses of ethanol extract EDB1 2.8 mg/20g bw; EDB2 5.6 mg/20g bw; EDB3 8.4 mg/20g bw, EDB4 11.2 mg/20g bw. After oral administration and a one-hour interval, the mice were placed on a rotarod and the duration of their endurance was observed. Statistical analysis showed that the endurance duration of the mice given EDB significantly differed from the negative control, indicating that EDB has a stimulant effect. EDB1 showed no significant difference with caffeine, implying its stimulant effect is comparable to caffeine. Meanwhile, EDB2, EDB3, and EDB4 showed higher endurance duration and significant differences with caffeine, implying that these EDB doses have a stronger stimulant effect than caffeine. However, there was no significant difference between EDB3 and EDB4.