Abstract
The aim of this study is to investigate antioxidant activity and total phenolic content of ethyl acetate fraction of Sembung leaves. Sembung (Blumea balsamifera L.) is a plant that has the potential as an antioxidant because it contains secondary metabolites of alkaloids, steroids, flavonoids, saponins, and phenolic compounds. In this study, antioxidant activity was tested by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) for free radical scavenging activity assay. Evaluation of total phenolic content was tested by using the Folin-Ciocalteau method. The results showed that the ethyl acetate fraction of Sembung leaves possess showed medium antioxidant activity with IC50 valueof 221.821 compare with reference standard ascorbic acid with IC50 value 68.25 ppm. The ethyl acetate fraction of Sembung leaves has a total phenolic content of 161.101 mg GAE/g. This study provided that Blumea balsamifera leaves possess antioxidant.
Highlights
The aim of this study is to investigate antioxidant activity and total phenolic content of ethyl acetate fraction of Sembung leaves
The results showed that the ethyl acetate fraction of Sembung leaves possess showed medium antioxidant activity with IC50 value of 221.821 compare with reference standard ascorbic acid with IC50 value 68.25 ppm
This study provided that Blumea balsamifera leaves possess antioxidant
Summary
The aim of this study is to investigate antioxidant activity and total phenolic content of ethyl acetate fraction of Sembung leaves. Tujuan dari penelitian ini adalah untuk melihat bagaimana aktivitas antioksidan dan kadar fenolik total, dari daun Sembung (Blumea balsamifera L.) yang tumbuh di daerah Sumatra Barat. Penetapan kadar fenolik total Sebanyak 5 mg fraksi etil asetat daun Sembung dilarutkan dengan etanol 96% dalam labu ukur 50 ml sehingga diperoleh larutan dengan konsentrasi 100 ppm.
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