Uji Aktivitas Antijamur Ekstrak Gambir (UncariagambirRoxb) terhadap Candida albicans secara in Vitro

Abstract

Effective treatment is being sought for the discovery of antifungal compounds.One of Plants that are suspected as a potential antifungal is Gambir (Uncaria gambierRoxb.). Gambir contains high antioxidants. Gambier containing catechin, katekutannat, quercetin, gallic acid, and catechol elagat acid, has been studied to determine its antifungal activity. This study was done by using experimental in vitro tube dilution method. The general objective of this study was to investigate the inhibition of ethanolic extract and water extract of gambier towards the growth of Candida albicans. Specific objectives were to determine Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC)of ethanolic extract and water extract of gambier towards the growth of Candida albicans. The concentration used were 50% , 60% , 70% , 80% , 90% and 100% . The results showed thatMICof the ethanolic extract of gambier could not be determined, while the MBCwas 100%. One Way ANOVA statistical analysis results showed p= 0.004 ( p< 0.05 ), indicating significant differences in changes of the concentration of ethanol extract gambier to the number of colonies ofCandida albicans. Correlation test showed a close relationship between the concentration of gambier extract and the number of colonies ( r = -9.900 : p < 0.05 ). Regression test yielda linear regression equation Y = 5,034-0,048x with R Square coefficient ( r2 ) of 0.802. Based on the research results it can be concluded that the ethanolic extract of gambier has antifungal effect against Candida albicans.