Abstract
The stew of Mirabilis jalapa L. flower has been used traditionally as an anti-inflammatory. Phytochemical screening of Mirabilis jalapa L. flower showed flavonoid content which is known to have anti-inflammatory activity. This study aims to determine the anti-inflammatory activity of Mirabilis jalapa L. flower infusion using the red blood cell membrane stabilization method by inducing hypotonicity and heat. The test group consisted of Mirabilis jalapa L. flower infusion with a concentration of 2.5%; 5%; and 10% w / v, positive control (diclofenac sodium 0.01% w / v and aspirin 0.01% w / v), and negative control (distilled water). The anti-inflammatory activity of Mirabilis jalapa L. flower infusion was determined by the percentage of membrane stabilization and inhibition of red blood cell hemolysis. Data from the test results were statistically analyzed both parametric and nonparametric with SPSS. Mirabilis jalapa L. flower infusion contains flavonoids, tannins and terpenoids. The test results showed that the 10% concentration of Mirabilis jalapa L. flower infusion had the largest percentage of red blood cell hemolysis protection on hypotonicity induction, that is 99.50% (p>0.05) and the largest percentage of hemolysis inhibition on heat induction, that is 50.27% ( p≤0.05). From these results, it can be concluded that the Mirabilis jalapa L. flower infusion has potential as an anti-inflammatory by stabilizing the red blood cell membrane.
Highlights
The stew of Mirabilis jalapa L. flower has been used traditionally as an antiinflammatory
The test results showed that the 10% concentration of Mirabilis jalapa L. flower infusion had the largest percentage of red blood cell hemolysis protection on hypotonicity induction, that is 99.50% (p>0.05) and the largest percentage of hemolysis inhibition on heat induction, that is 50.27% (p≤0.05)
It can be concluded that the Mirabilis jalapa L. flower infusion has potential as an antiinflammatory by stabilizing the red blood cell membrane
Summary
Aktivitas Antiinflamasi Infusa Bunga Pukul Empat (Mirabilis jalapa L.) Secara In Vitro. Rebusan bunga pukul empat (Mirabilis jalapa L.) telah digunakan sebagai antiradang secara tradisional. Skrining fitokimia bunga Mirabilis jalapa L. menunjukkan kandungan flavonoid yang diketahui memiliki aktivitas antiinflamasi. Penelitian ini bertujuan untuk menentukan aktivitas antiinflamasi infusa bunga Mirabilis jalapa L. menggunakan metode stabilisasi membran sel darah merah dengan induksi hipotonisitas dan panas. Aktivitas antiinflamasi infusa bunga Mirabilis jalapa L. ditentukan melalui persentase stabilisasi membran dan inhibisi hemolisis sel darah merah. Hasil uji menunjukkan bahwa infusa bunga Mirabilis jalapa L. konsentrasi 10% memiliki persentase proteksi hemolisis sel darah merah terbesar pada induksi hipotonisitas yakni 99,50% (p>0,05) dan persentase inhibisi hemolisis terbesar pada induksi panas yakni 50,27% (p≤0,05). Dari hasil tersebut dapat disimpulkan bahwa infusa bunga Mirabilis jalapa L. memiliki potensi sebagai antiinflamasi dengan stabilisasi membran sel darah merah.
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