Abstract
Dayak onion (Eleutherine polmifolia (L.) Merr) was a typical plant of Kalimantan. Empirically dayak onion (Eleutherine polmifolia (L.) Merr) used by the comunity to treat skin infections. The purpose of this study was to determine the antibacterial activity of ethanol extract of dayak onion power against Salmonella typhi and Escherichia coli bacteria. The highest concentration used was 100%, and the lowest concentration was 0,5%. As a control of cloramfenikol for Salmonella typhi and ciprofloxacin for Escherichia coli bacteria. This research method used agar diffusion. The results showed the ethanol extract of dayak onion (Eleutherine polmifolia (L.) Merr) contained flavonoids, alkaloids, tannins and phenols with the highest levels was by tannins at 7,45%. Dayak onion (Eleutherine polmifolia (L.) Merr) are inhibiting the growth of Salmonella typhi bacteria at a minimum inhibitory concentration (MIC)of 1% with a inhibition zone diameter of 6,24 mm and 0,9% with a inhibition zone diameter of 6,20 mm at Escherichia coli bacteria.Keywords : Eleutherine polmifolia (L.) Merr, Salmonella typhi, Escherichia coli, Agar diffusion, MIC
Highlights
Dayak onion (Eleutherine polmifolia (L.) Merr) was a typical plant of Kalimantan
The results showed the ethanol extract of dayak onion (Eleutherine polmifolia (L.) Merr) contained flavonoids, alkaloids, tannins and phenols with the highest levels was by tannins at 7,45%
W., Wardani, S.D., Ramadhan, A.A. dan Yuliani, R., 2018
Summary
Alat yang digunakan pada penelitian ini yaitu oven, lemari pendingin, Hotplate, Autoclave, jarum ose, pinset, lidi kapas steril, cawan petri, Erlenmeyer, Beaker glass, pipet ukur, bulp, kertas kopi, api spiritus, inkubator, tabung reaksi dan rak tabung, Paper disc, jangka sorong, batang pengaduk (spatula), neraca analitik, pisau, bejana, seperangkat alat perkolasi, kapas, plastik, kertas saring, spektrofotometri. Bahan yang digunakan dalam penelitian ini yaitu bawang dayak, disk Kloramfenikol, disk Ciprofloxasin, media Salmonella Shigella Agar (SSA), media Triptyc Soy Agar (TSA), media Natrium Agar (NA), aquades, kertas cakram, biakan bakteri Salmonella typhi dan Escherichia coli koleksi Laboratorium Teknik Hasil Pertanian Politeknik Negeri Lampung, standar Mac Farland ( BaCl2 1% : H2SO4 1% ), etanol 96%
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