Abstract
BackgroundUp-regulation of UHRF1 has been observed in a variety of cancers and appears to serve as an independent prognostic factor.ObjectiveTo explore the effect of UHRF1 gene silencing on apoptosis and proliferation of cervical squamous cell carcinoma (CSCC) CaSki cells.MethodsThis study consisted of 47 CSCC tissues and 40 normal cervical tissues. The CaSki cells were assigned into Blank group (CaSki cells not transfected), NC group (CaSki cells transfected with control siRNA), and UHRF1 Silence group (CaSki cells transfected with UHRF1 siRNA). qRT-PCR and Western blot were used for UHRF1 mRNA and protein expressions, CKK-8 assay for cell proliferation, flow cytometry for cell cycle and apoptosis, Western blot for expressions of apoptosis-related proteins. Nude mice tumor transplant experiment was performed.ResultsUHRF1 exhibited higher mRNA and protein expressions in the CSCC tissues than normal cervical tissues (both P < 0.05). The cell proliferation ability in the UHRF1 Silence group was reduced when compared with the Blank group and the NC group, the cells at S-G2M stage in the UHRF1 Silence group were dropped when compared with the Blank group and the NC group (P < 0.05), while the cells at G0/G1 stage were elevated (P < 0.05), and the proportion of Annexin V positive cells in the UHRF1 Silence group was increased in comparison with the Blank group and the NC group (P < 0.05). Nude mice tumor transplant experiment indicated that the growth rate and weight of tumor in the Blank group and NC group was higher and heavier than the UHRF1 Silence group (P < 0.05).ConclusionUHRF1 showed a high expression in CSCC and UHRF1 silencing can reduce proliferation and enhance apoptosis of the CaSki cells.
Highlights
Up-regulation of UHRF1 has been observed in a variety of cancers and appears to serve as an independent prognostic factor
The cells were classified into 3 groups: the Blank group (CaSki cells untransfected), the negative control (NC) group (CaSki cells transfected with control siRNA) and the UHRF1 Silence group (CaSki cells transfected with UHRF1 siRNA)
The findings confirmed that UHRF1 transcription level was promoted in the cervical squamous cell carcinoma (CSCC) tissues when compared with the normal tissues and it could be inhibited by UHRF1 siRNA in the CaSkin cell lines
Summary
Up-regulation of UHRF1 has been observed in a variety of cancers and appears to serve as an independent prognostic factor. UHRF1 [ubiquitin-like, containing plant homeodomain (PHD) and really interesting new gene (RING) finger domains 1], known as ICBP90 or Np95, is a member of UHRF family and encode a 95-kDa nuclear protein of 793 amino acids, with a single open reading frame (ORF) [5]. It contains an N-terminal ubiquitylation-like domain, PHD, a SRA (SET and RING-associated) domain and a RING finger motif domain [6]. The present study aims at exploring the effects of silent UHRF1 on proliferation and apoptosis of human CSCC Caski cells and to further study the related mechanism of cell apoptosis
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