Abstract
To identify metabolic biomarkers related to the freshness of chilled chicken, ultra-high-performance liquid chromatography–mass spectrometry (UHPLC–MS/MS) was used to obtain profiles of the metabolites present in chilled chicken stored for different lengths of time. Random forest regression analysis and stepwise multiple linear regression were used to identify key metabolic biomarkers related to the freshness of chilled chicken. A total of 265 differential metabolites were identified during storage of chilled chicken. Of these various metabolites, 37 were selected as potential biomarkers by random forest regression analysis. Receiver operating characteristic (ROC) curve analysis indicated that the biomarkers identified using random forest regression analysis showed a strong correlation with the freshness of chilled chicken. Subsequently, stepwise multiple linear regression analysis based on the biomarkers identified by using random forest regression analysis identified indole-3-carboxaldehyde, uridine monophosphate, s-phenylmercapturic acid, gluconic acid, tyramine, and Serylphenylalanine as key metabolic biomarkers. In conclusion, our study characterized the metabolic profiles of chilled chicken stored for different lengths of time and identified six key metabolic biomarkers related to the freshness of chilled chicken. These findings can contribute to a better understanding of the changes in the metabolic profiles of chilled chicken during storage and provide a basis for the further development of novel detection methods for the freshness of chilled chicken.
Highlights
Due to its advantages of high protein, low cholesterol and low fat, chicken is one of the most traded and consumed meats worldwide [1]
We find that the relative content of tyramine is low during the first one to three days and increases significantly on Day 5 (p < 0.05), suggesting that tyramine could be an important biomarker related to the freshness of chicken
The present study provides new insightsinto intothe theidentification identificationofofmetabolic metabolicbiomarkers biomarkers related related to thetofreshness of chilled chicken
Summary
Due to its advantages of high protein, low cholesterol and low fat, chicken is one of the most traded and consumed meats worldwide [1]. The demand for chilled chicken has markedly increased, and the safety problems of chilled chicken have become a public health concern [3].
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