UHPLC-ESI-qTOF-MS Analysis of Cyclopeptide Alkaloids in the Seeds of Ziziphus jujuba var. spinosa

Kyo Bin Kang,Dae Sik Jang,Sang Hyun Sung,Jinwoong Kim

doi:10.5478/msl.2016.7.2.45

Abstract

An UHPLC-ESI-qTOF-MS analytical method was developed for cyclopeptide alkaloids in the seeds of Ziziphus jujuba var. spinosa (Semen Ziziphi Spinosae), which is a commonly used herb in Chinese and Korean traditional medicines. Considering the basicity of cyclopeptide alkaloids, a specific separation method was developed for an UHPLC system. The compounds were detected by DAD and ESI-qTOF-MS, and their fragmentation patterns were also acquired by MSE technologies. Peak-picking of major compounds was performed with nine previously isolated compounds and two reference standard compounds. Tandem MS fragmentation behaviors of type-Ia and -Ib cyclopeptide alkaloids were investigated with the acquired data to develop a strategy for dereplication of other cyclopeptide alkaloid compounds in Z. jujuba var. spinosa. Two more cyclopeptide alkaloids were tentatively identified with UHPLC–ESI-qTOF-MS using this method.

Highlights

In recent screenings of natural products by chemists, more cyclopeptide alkaloids have been discovered in marine organisms than in plants
An UHPLC-ESI-qTOF-MS analytical method was developed for cyclopeptide alkaloids in the seeds of Ziziphus jujuba var. spinosa (Semen Ziziphi Spinosae), which is a commonly used herb in Chinese and Korean traditional medicines
Tandem MS fragmentation behaviors of type-Ia and -Ib cyclopeptide alkaloids were investigated with the acquired data to develop a strategy for dereplication of other cyclopeptide alkaloid compounds in Z. jujuba var. spinosa

Summary

Introduction

In recent screenings of natural products by chemists, more cyclopeptide alkaloids have been discovered in marine organisms than in plants. Abstract : An UHPLC-ESI-qTOF-MS analytical method was developed for cyclopeptide alkaloids in the seeds of Ziziphus jujuba var. Two more cyclopeptide alkaloids were tentatively identified with UHPLC–ESI-qTOF-MS using this method. Keywords : Cyclopeptide alkaloids, UHPLC–ESI-qTOF-MS, Ziziphus jujuba var.

Results

Conclusion