UHPLC-MS Analysis of Polyphenols in the Aqueous Extract of Hydrangea seemannii

Abstract

Chemical analysis of the ethyl acetate soluble fraction prepared from the infusion of Hydrangea seemannii L. Riley, Hydrangeaceae, an endemic medicinal plant of Mexico, afforded thirteen polyphenols identified as chlorogenic acid (1), 3,5- and 4,5-dicaffeoylquinic acids (2 and 3), and ten flavonoids (4–13). Of these, the isolation of guajaverin (6) and juglalin (7) are reported for the first time in the Hydrangea genus. The structures were elucidated on basis of the spectroscopic and spectrometric data. Next, a selective LC-MS method was developed for the simultaneous determination of five flavonoids in the ethyl acetate soluble fraction and then validated according to the ICH guidelines (2005). Finally, the developed LC-MS method was successfully applied to quantify the flavonoids content of 4 (3.69 mg/g), 6 (3.83 mg/g), 7 (5.84 mg/g), 8 (2.30 mg/g), and 12 (1.40 mg/g).