Abstract
BackgroundThe transcribed ultraconserved regions (T-UCRs) are a novel class of non-coding RNAs that are absolutely conserved across species and are involved in carcinogenesis in some cancers. However, the expression and biological role of T-UCRs in renal cell carcinoma (RCC) remain poorly understood. This study aimed to examine the expression and functional role of Uc.416 + A and analyze the association between Uc.416 + A and epithelial-to-mesenchymal transition in RCC.MethodsExpression of Uc.416 + A in 35 RCC tissues, corresponding normal kidney tissues and 13 types of normal tissue samples was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). We performed a cell growth and migration assay in RCC cell line 786-O transfected with negative control and siRNA for Uc.416 + A. We evaluated the relation between Uc.416 + A and miR-153, which has a complimentary site of Uc.416 + A.ResultsqRT-PCR analysis revealed that the expression of Uc.416 + A was higher in RCC tissues than that in corresponding normal kidney tissues. Inhibition of Uc.416 + A reduced cell growth and cell migration activity. There was an inverse correlation between Uc.416 + A and miR-153. Western blot analysis showed Uc.416 + A modulated E-cadherin, vimentin and snail. The expression of Uc.416 + A was positively associated with the expression of SNAI1, VIM and inversely associated with the expression of CDH1.ConclusionsThe expression of Uc.416 + A was upregulated in RCC and especially in RCC tissues with sarcomatoid change. Uc.416 + A promoted epithelial-to-mesenchymal transition through miR-153. These results suggest that Uc.416 + A may be a promising therapeutic target.
Highlights
The transcribed ultraconserved regions (T-UCRs) are a novel class of non-coding RNAs that are absolutely conserved across species and are involved in carcinogenesis in some cancers
We examined the expression of Uc.416 + A in renal cell carcinoma (RCC) tissues, investigated its functional role of Uc.416 + A in RCC progression and analyzed its involvement in epithelial-to-mesenchymal transition (EMT)
Expression of Uc.416 + A is upregulated in RCC tissues We compared the expression of Uc.416 + A in 35 RCC tissues and corresponding normal kidney tissues by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and considered a RCC tissue/normal kidney tissue ratio > 2.0 as upregulation
Summary
The transcribed ultraconserved regions (T-UCRs) are a novel class of non-coding RNAs that are absolutely conserved across species and are involved in carcinogenesis in some cancers. The expression and biological role of T-UCRs in renal cell carcinoma (RCC) remain poorly understood. Transcribed ultraconserved regions (T-UCRs) are novel class of ncRNAs which are highly conserved among most of the vertebrate genomes [7]. Sekino et al BMC Cancer (2018) 18:952 at both fragile sites and cancer-associated genomic regions [8], indicating that T-UCRs are believed to play critical roles in human cancer. It appears that some T-UCRs serve as oncogenes or tumor suppressor genes in some cancers [9, 10]. Little is known about the expression and biological role of T-UCRs including Uc.416 + A in RCC
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