Abstract
Cutaneous squamous cell carcinoma is prone to metastasis at its advanced stage. Previous studies reported that the ubiquitin-conjugating enzyme E2C is a proto-oncogene that causes cancer in various tumors. This study aimed to investigate the role of the ubiquitin-conjugating enzyme E2C in cutaneous squamous cell carcinoma. We initially analyzed the expression profile in the gene expression omnibus database. We obtained the intersection genes, established a protein-protein interaction network, and identified ubiquitin-conjugating enzyme E2C as the key gene. Furthermore, microarray data revealed that the ubiquitin-conjugating enzyme E2C expression is elevated in cutaneous squamous cell carcinoma. Ubiquitin-conjugating enzyme E2C overexpression plasmids and siRNA of ubiquitin-conjugating enzyme E2C were transfected into cutaneous squamous cell carcinoma cells to analyze the effects of ubiquitin-conjugating enzyme E2C on cutaneous squamous cell carcinoma cell proliferation, migration, and invasion. The expression of associated proteins was detected using Western blot assay to confirm the impact of the ubiquitin-conjugating enzyme E2C on the phosphatidylinositol-3-kinase/threonine kinase signaling pathway. Ubiquitin-conjugating enzyme E2C knockdown inhibited cutaneous squamous cell carcinoma cell viability, migration, and invasion, and the phosphatidylinositol-3-kinase/threonine kinase signaling pathway, while ubiquitin-conjugating enzyme E2C overexpression had opposite results. In summary, Ubiquitin-conjugating enzyme E2C is a potential therapeutic target for cutaneous squamous cell carcinoma since it encourages the proliferation and migration of cutaneous squamous cell carcinoma cells.
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