Abstract
Microbial products play a role in the pathogenesis of allergic diseases; ubiquitin E3 ligase A20 (A20) is an important molecule in regulating inflammation in the body. The present study aims to elucidate the role of A20 in processing the absorbed microbial products in nasal epithelial cells. Human nasal mucosal specimens were collected from patients with or without chronic rhinitis and analyzed by immunohistochemistry. Human nasal epithelial cell line, RPMI2650 cell, was employed to assess the role of A20 in processing the absorbed staphylococcal enterotoxin B (SEB). The RPMI2650 cells absorbed SEB in the culture. The increase in A20 was observed in RPMI2650 cells in parallel to the absorption of SEB. A20 is a critical molecule in the degradation of SEB in the nasal epithelial cells by promoting the tethering of endosomes and lysosomes. A20 plays a critical role in processing of the absorbed SEB in nasal epithelial cells.
Highlights
The results indicate that nasal epithelial cells endocytosed staphylococcal enterotoxin B (SEB), which increased the expression of ubiquitin E3 ligase A20
C, the cells were cultured in the presence of fluorescein isothiocyanate (FITC)-labeled SEB (10 g/ml) and collected at the indicated time points; the cells were smeared on slides and observed with a confocal microscope
The present data reveal a novel mechanism by which nasal epithelial cells process the absorbed microbial product, SEB
Summary
Results: A20 facilitates endosome/lysosome fusion and protein degradation in epithelial cells. Significance: A20 is a critical molecule in maintaining the homeostasis in the body. Microbial products play a role in the pathogenesis of allergic diseases; ubiquitin E3 ligase A20 (A20) is an important molecule in regulating inflammation in the body. The present study aims to elucidate the role of A20 in processing the absorbed microbial products in nasal epithelial cells. Human nasal epithelial cell line, RPMI2650 cell, was employed to assess the role of A20 in processing the absorbed staphylococcal enterotoxin B (SEB). A20 is a critical molecule in the degradation of SEB in the nasal epithelial cells by promoting the tethering of endosomes and lysosomes. A20 plays a critical role in processing of the absorbed SEB in nasal epithelial cells
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