Abstract
The objective of the investigation was to evaluate the percutaneous penetration of a ubidecarenone-loaded nanostructured lipid carrier (UB-NLC) and to illuminate the protective effects of UB-NLC for amelioration of hydrogen peroxide-induced oxidative damage on HaCaT cells. Ubidecarenone (UB) was encapsulated in a nanostructured lipid carrier (NLC), which was manufactured by homogenization. The morphological and dimensional properties of the prepared UB-NLC were studied by freeze-fracture transmission electron microscopy (FF-TEM) and photon correlation spectroscopy (PCS). Percutaneous penetration of UB-NLC was carried out by the Franz diffusion cells method. The change of cellular morphology was identified through a non-invasive time-lapse imaging system. The assessment was achieved via the evaluation of the levels of oxidative stress markers: reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and malondialdehyde (MDA). Percutaneous penetration of UB loaded in NLC formulation was enhanced in comparison to free UB. Preincubation of HaCaT cells with UB-NLC attenuated the level of intracellular generation of ROS. Lipid peroxidation was diminished by UB-NLC via inhibition of MDA formation. Pretreatment of cells with UB-NLC reestablished the activity of cellular antioxidant enzymes (SOD and GSH-PX). On the basis of the investigation conducted, results suggest that formulating UB as NLC is advantageous for topical delivery and treatment of oxidative stress-induced human diseases.
Highlights
Reactive oxygen species (ROS) are engendered in the oxidation-reduction procedure [1]
Ubidecarenone was successfully encapsulated into a nanostructured lipid carrier by high pressure homogenization, preparing a stable and topically attractive aqueous formation (UB-NLC)
The attained results highlight that NLC is a good nanocarrier for ubidecarenone
Summary
Reactive oxygen species (ROS) are engendered in the oxidation-reduction procedure [1]. ROS, as products of the oxidation and peroxidation process, are instantaneously detoxified by ROS scavengers and antioxidants under physiological conditions [2]. Types of ROS, such as superoxide radical, hydrogen peroxide, hydroxyl radical, are generated in aerobic living organisms and are present in the cell under balance with oxidation and antioxidation procedure [3]. OS is induced by an overproduction of ROS, which may bring about direct or indirect ROS-mediated oxidative impairment, for example, disruption of the cellular membrane, changes in nucleic acids, protein denaturation, and lipid peroxidation; it has been implicated in cells apoptosis and physiological dysfunction [4,8,9,10,11,12]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
