Abstract
The ability of phytochemicals to suppress production of superoxide anion (O 2 −̇) in a cell culture model was assessed. Differentiated U937 cells and human polymorph nuclear leukocytes (PMN) were stimulated with increasing concentrations of phorbol myristate acetate (PMA) and O 2 −̇ release was measured by cytochrome c reduction. The overall trend in O 2 −̇ production in response to PMA stimulation was similar in both U937 cells and PMN. The effect of the phytochemicals, chlorogenic acid, caffeic acid, curcumin, quercetin, (-)-epicatechin and glycyrrhizic acid (50, 100 and 500 nM) on O 2 −̇ formation from PMA stimulated U937 cells was examined. All phytochemicals suppressed O 2 −̇ release from U937 cells with chlorogenic acid, (-)-epicatechin and glycyrrhizic acid showing dose dependent effects. In conclusion, U937 cells are a useful model to screen phytochemicals for their ability to suppress O 2 −̇ formation. © 1997 Elsevier Science Inc.
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