Abstract
BackgroundMedicinal plants are becoming more popular in the treatment of various diseases because of the adverse effects of the current therapy, especially antioxidant plant components such as phenols and flavonoids have a protective role against oxidative stress-induced degenerative diseases like diabetes. Thus, the purpose of this study was to investigate β-cell protection and antidiabetic activities of Crassocephalum crepidioides (Asteraceae) Benth. S. Moore.MethodThe in-vitro study was conducted by the pancreatic β-cell culture and α-amylase inhibition technique which includes two methods, namely starch-iodine method and 3,5-dinitrosalicylic acid (DNSA) method. On the other hand, the in-vivo study was performed by oral glucose tolerance test (OGTT) method and alloxan-induced diabetes method by using Wistar albino rat. At the end pancreatic specimens were removed and processed for histopathological study.ResultThe plant extract showed significant (*p < 0.05, **p < 0.01) effect on hyperglycemia as compared to standard (Gliclazide) in OGTT. The plant extract showed efficient protection activity of pancreatic β-cell from cell death in INS-1 cell line by significantly reduced (*p < 0.05, **p < 0.01) the levels alloxan-induced apoptosis and intracellular reactive oxygen species (ROS) accumulation. In addition, the plant extract showed a significant (*p < 0.05, **p < 0.01) effect on hyperglycemia by increases in percent of β-cells present in each islet (45% – 60%) compared to the diabetic group.ConclusionThe result showed that C. crepidioides had β-cell protection and antidiabetic activities in pancreatic β-cell culture and Wistar albino rat.
Highlights
Medicinal plants are becoming more popular in the treatment of various diseases because of the adverse effects of the current therapy, especially antioxidant plant components such as phenols and flavonoids have a protective role against oxidative stress-induced degenerative diseases like diabetes
Phenols and flavonoids Phytochemical assay by chromatographic method showed that the main components, including phenolic and flavonoid compounds were present in the extract (Table 1)
The plant extract showed significant % inhibition of α-amylase as compared to the standard anti-diabetic drug, acarbose, in both starch-iodine test and dinitrosalicylic acid (DNSA) assay that could be attributed to the presence of phenolic and flavonoids components (Table 1) because polyphenols are capable of inhibiting carbohydrate hydrolyzing enzymes due to their ability to bind with proteins [31, 32]
Summary
Medicinal plants are becoming more popular in the treatment of various diseases because of the adverse effects of the current therapy, especially antioxidant plant components such as phenols and flavonoids have a protective role against oxidative stress-induced degenerative diseases like diabetes. Diabetes mellitus is a metabolic disorder characterized by high blood sugar (hyperglycemia) due to the disturbances of carbohydrate, fat and protein metabolism [1]. It can be classified as type-1 diabetes known as insulin-dependent diabetes mellitus (IDDM) and type-2 diabetes called non-insulin dependent diabetes mellitus (NIDDM) [2]. Alloxan, is a potent diabetogenic agent that selectively damage pancreatic β-cells and it has been widely used as diabetes inducer [5]. Recent studies have demonstrated that medicinal plants can protect β-cell by reducing alloxan-induced oxidative stress by scavenging ROS in-vitro and in-vivo, respectively [12,13,14]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
