Abstract
The biosynthetic potential of soil-dwelling actinomycetes to produce diverse bioactive molecules that are useful as drug seeds has been achieved in the laboratory by modifying culture conditions. Availability of a small molecule that can induce secondary metabolism in these microbes can greatly facilitate the exploration of bioactive natural products. In this manuscript, through the screening of natural products and chemical modification, we demonstrated that the presence of the β-carboline compound, BR-1, enhanced reveromycin A production in Streptomyces sp. SN-593. BR-1 induced reveromycins production at the wide range of concentrations without affecting cell growth. Our study indicates that BR-1 might serve as an alternative to activate specialized metabolite biosynthesis without genetic engineering.
Highlights
Soil dwelling actinomycetes are characterized by their ability to produce diverse specialized metabolites (SMs), that account for majority of drugs presently in clinical use[1]
We defined extracellular chemical signals as biomediators to distinguish them from autoregulators
After the failed attempt of purification from tomato juice, we utilized the antifungal activity of RM-A to screen overproduced RMs after the treatment of Natural Products Depository (NPDepo) compounds
Summary
Soil dwelling actinomycetes are characterized by their ability to produce diverse specialized metabolites (SMs), that account for majority of drugs presently in clinical use[1]. The activity of putative hits was further analysed by the srcts-NRK cells assay followed by liquid chromatography-mass spectrometry (LC-MS) analysis to quantify the production of RM-A (1) and its derivative RM-B (2). Structural insights into the biomediator activity of β-carbolines.
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