U-DCS: characterization of the first permanent human dendritic sarcoma cell line

Kevin Mellert,Winfried Barchet,Daniel Fürst,Joannis Mytilineos,Julian Benckendorff,Regine Mayer-Steinacker,Michaela Buck,Andreas Viardot,Peter Wiegand,Katarzyna Zimmermann,Gunther Hartmann,Peter Möller,Frank Leithäuser,Muriel Malaise,Giada Frascaroli

doi:10.1038/s41598-020-77471-7

Kevin Mellert, Winfried Barchet + Show 13 more

Open Access

https://doi.org/10.1038/s41598-020-77471-7

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Abstract

A dendritic cell sarcoma cell line, U-DCS, was established from a dendritic cell sarcoma in a 53-year-old Caucasian male patient. Since its establishment, U-DCS has maintained stable phenotypic characteristics in vitro and has a doubling time of approximately 2 days under standard culture conditions. U-DCS is growing with typical dendritic cell morphology in tissue and expresses the dendritic cell sarcoma immunophenotypic markers S100 protein, MHCI, MHCII, and vimentin. Expression analysis revealed transcripts for the toll-like receptors TLR3, -4, -9 and DDX58 (RIG-I), but not for TLR2. U-DCS shows functional features of dendritic cells with the ability of phagocytosis and antigen-specific T cell stimulation. Karyotype-, CGH-, and mFISH analysis point to a chromosomal instability and a hypotetraploid karyotype with approximately 130 chromosomes. U-DCS is the first immortalized human dendritic cell sarcoma cell line and has some morphological and functional features of dendritic cells without dependency on growth factors.

Highlights

A dendritic cell sarcoma cell line, U-DCS, was established from a dendritic cell sarcoma in a 53-yearold Caucasian male patient
Our results demonstrate that we successfully established a dendritic cell sarcoma cell line, U-DCS, and that U-DCS cells bear morphological and functional resemblance to IDC sarcoma (IDCS) and to some extent to conventional dendritic cells
U-DCS is the first human permanent dendritic cell sarcoma cell line derived from an IDCS

Summary

Introduction

A dendritic cell sarcoma cell line, U-DCS, was established from a dendritic cell sarcoma in a 53-yearold Caucasian male patient. U-DCS shows functional features of dendritic cells with the ability of phagocytosis and antigen-specific T cell stimulation. U-DCS is the first immortalized human dendritic cell sarcoma cell line and has some morphological and functional features of dendritic cells without dependency on growth factors. Paracortical T-cell areas harboring DCs in lymph nodes have been referred to as interdigitating dendritic cells (IDC) by h istologists[3,4]. A pooled analysis of dendritic cell sarcoma by Saygin et al in 2013 identified 100 IDCS cases in the English literature[12]. We here report the first permanent human IDCS cell line, U-DCS, derived from an IDCS of a male Caucasian patient. Serial passaging of the cells gave rise to a rapidly proliferating cell line, which was characterized by immunocytochemical, molecular, and cytogenetic techniques and was functionally tested for phagocytosis and antigen presentation

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