Tyrosine hydroxylase- and/or aromatic l-amino acid decarboxylase-containing cells in the suprachiasmatic nucleus of the Syrian hamster ( Mesocricetus auratus)

Abstract

Catecholamines, including dopamine (DA), affect the activity of cells in the suprachiasmatic nucleus (SCN) of the hypothalamus, the principal circadian clock in mammals. This study examined the distribution of dopaminergic cells in the SCN of the male Syrian hamster, using both single- and double-label immunocytochemistry for tyrosine hydroxylase (TH), the rate-limiting enzyme in DA synthesis and for aromatic l-amino acid decarboxylase (AADC), the second enzyme needed to produce DA. Some neurons immunopositive for TH (TH+) were found in the SCN, but most of the TH+ cells of the region were located just outside the borders of the nucleus, as defined by pyronin Y staining. In the SCN, 91% of these cells were also immunopositive for AADC and thus, likely to be dopaminergic. Cells positive for AADC, many of which were not TH+, were found throughout the SCN, with the highest concentration seen in the ventral aspects of the nucleus. Cells containing AADC, but lacking TH may synthesize products other than DA, such as trace amines. These anatomical observations suggest that local neurons that produce DA and perhaps trace amines, may play a role in SCN function and in the neural control of circadian rhythms.