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Abstract
The change in tyrosinase activity in silkworm blood from the full-grown larval stage to pupation follows a V-shaped curve. In the integument the curve is almost reversed, and the activity is lowest just after pupation. Tyrosinase activity in the blood plasma gives an almost similar curve to that of whole blood, whereas in the haemocytes the activity is rather constant and the ratio of the activity in blood plasma to whole blood was very low at pupation compared with any other time. Tyrosinase could be observed only in oenocytoids (and/or phagocytes of the pupal type); the other types of haemocytes scarcely stained with Nadi reagent or catechol solution. During pupation the darkly stained haemocytes amount to several per cent of all haemocytes. The change of tyrosinase activity in acetone powder of the blood is fairly different from that of intact blood, and the amount of copper found in blood plasma is higher than in the whole blood during pupation.
Published Version
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