TYPE VB AND VI SECRETION SYSTEMS AS COMPETITION AGENTS OF GRAM-NEGATIVE BACTERIA

Abstract

Abstract Bacterial competition, defined as a local neighbour interactions, can lead to competitors coexistence, bacterial community self-organization or as travelling waves of species dominance in ecological niches. Bacteria have developed many mechanisms to communicate and compete. Kin discrimination mechanisms in bacterial populations allow species to distinguish a friend from a foe in bacterial environment. Type Vb and VI secretion systems (TVIbSS and TVISS) play crucial role in this phenomenon. A contact-dependent growth inhibition (CDI), primarily found in Escherichia coli strains, utilities CdiB/CdiA protein of type Vb secretion system, described also as two-partner secretion (TPS) system, to inhibit growth of non-kin strains, where cell contact is required. Presence of an intracellular small immunity protein (CdiI) protects E. coli cells from autoinhibition. Other bacterial competition system, primarily found in nodulation process of Rhizobium leguminosarum bv. Trifolii strain, engages type VI secretion system. The structure of TVISS is more complicated and comprises the series of proteins with structural homology to bacteriophage tail proteins and membrane proteins which builds the core of the system (Tss proteins). Meanwhile, other proteins of the TVISS was described as associated proteins (Tag proteins). Important proteins for TVISS are haemolysin coregulated protein (Hcp) which has hexameric, tubular structure and VgrG protein (valine-glycine repeat G) which play a dual role in the process: as a chaperone protein in secretion of effector toxin or/and as a secreted toxin itself. Despite the structural differences of both secretion systems they show functional homology in competition phenomenon and govern the social life of bacterial community. 1. Introduction. 2. Contact-dependent growth inhibition. 2.1. Structure o CDI machinery. 2.2. Effectors of CDI system. 3. Type VI secretion system. 3.1. Structure of type VI secretion system. 3.2. Effectors of type VI secretion system. 4. Membership to polymorphic toxins system. 5. Role of the systems in bacterial biology. 6. Conclusions