Type-specific dendritic integration in mouse retinal ganglion cells

Yanli Ran,Katrin Franke,Philipp Berens,Timm Schubert,Ziwei Huang,Harald Baayen,Thomas Euler,Tom Baden

doi:10.1038/s41467-020-15867-9

Abstract

Neural computation relies on the integration of synaptic inputs across a neuron’s dendritic arbour. However, it is far from understood how different cell types tune this process to establish cell-type specific computations. Here, using two-photon imaging of dendritic Ca2+ signals, electrical recordings of somatic voltage and biophysical modelling, we demonstrate that four morphologically distinct types of mouse retinal ganglion cells with overlapping excitatory synaptic input (transient Off alpha, transient Off mini, sustained Off, and F-mini Off) exhibit type-specific dendritic integration profiles: in contrast to the other types, dendrites of transient Off alpha cells were spatially independent, with little receptive field overlap. The temporal correlation of dendritic signals varied also extensively, with the highest and lowest correlation in transient Off mini and transient Off alpha cells, respectively. We show that differences between cell types can likely be explained by differences in backpropagation efficiency, arising from the specific combinations of dendritic morphology and ion channel densities.

Highlights

Neural computation relies on the integration of synaptic inputs across a neuron’s dendritic arbour
We studied dendritic integration in four types of mouse Off retinal ganglion cell (RGC), which have their dendrites in overlapping strata of the inner plexiform layer (IPL) and, receive highly overlapping sets of synaptic input
The more isolated, independent dendritic segments in tOff alpha cells may help them to detect fine structures of visual stimuli and support visual computations relying on spatial resolution below the receptive fields (RFs) of the entire cell

Summary

Introduction

Neural computation relies on the integration of synaptic inputs across a neuron’s dendritic arbour. We exploit the unique structure of the IPL to isolate the contributions of type-specific synaptic input profiles from intrinsic cellular mechanisms to elucidate whether RGC types sampling from a similar input space use specific dendritic integration profiles to generate functionally diverse outputs. To this end, we studied the dendritic integration properties of four Off RGC types in the mouse retina that receive excitatory input from a highly overlapping set of presynaptic neurons. A biophysical model suggests that the differential dendritic integration in these RGC types arises from the type-specific combination of dendritic morphology and ion channel complement

Methods

Results

Conclusion