Type of hormonal treatment administered to induce vitellogenesis in European eel influences biochemical composition of eggs and yolk-sac larvae

E Benini,J Tomkiewicz,S Engrola,A Nielsen,S.N Politis,S R Sørensen

doi:10.1007/s10695-021-01042-4

E Benini, J Tomkiewicz + Show 4 more

Open Access

https://doi.org/10.1007/s10695-021-01042-4

Copy DOI

Abstract

Egg biochemical composition is among the main factors affecting offspring quality and survival during the yolk-sac stage, when larvae depend exclusively on yolk nutrients. These nutrients are primarily embedded in the developing oocytes during vitellogenesis. In aquaculture, assisted reproduction procedures may be applied enabling gamete production. For the European eel (Anguilla anguilla), reproductive treatment involves administration of pituitary extracts from carp (CPE) or salmon (SPE) to induce and sustain vitellogenesis. In the present study, we compared the influence of CPE and SPE treatments on offspring quality and composition as well as nutrient utilization during the yolk-sac stage. Thus, dry weight, proximal composition (total lipid, total protein), free amino acids, and fatty acids were assessed in eggs and larvae throughout the yolk-sac stage, where body and oil-droplet area were measured to estimate growth rate, oil-droplet utilization, and oil-droplet utilization efficiency. The results showed that CPE females spawned eggs with higher lipid and free amino acid contents. However, SPE females produced more buoyant eggs with higher fertilization rate as well as larger larvae with more energy reserves (estimated as oil-droplet area). Overall, general patterns of nutrient utilization were detected, such as the amount of total lipid and monounsaturated fatty acids decreasing from the egg stage and throughout the yolk-sac larval stage. On the contrary, essential fatty acids and free amino acids were retained. Notably, towards the end of the yolk-sac stage, the proximal composition and biometry of surviving larvae, from both treatments, were similar.

Highlights

The production of good-quality eggs relies on the optimal progression of oogenesis, where the coordinated assembly of developing eggs is controlled by an interplay of endocrine and intra-ovarian factors, a process that can take a year or more (Tyler and Sumpter 1996)
The role of luteinizing hormone (LH) primarily relates to the final maturation of the follicle, stimulating the production of maturation-inducing hormone (MIH) and maturation-promoting factor (MPF), leading to ovulation (Nagahama and Yamashita 2008)
The present study assessed differences in quality and biochemical composition of European eel offspring produced via two alternative female treatment protocols (CPE vs. SPE)

Summary

Introduction

The production of good-quality eggs relies on the optimal progression of oogenesis, where the coordinated assembly of developing eggs is controlled by an interplay of endocrine and intra-ovarian factors, a process that can take a year or more (Tyler and Sumpter 1996). Pituitary gonadotropins regulate oogenesis through stimulation of sex steroid synthesis by follicle cells surrounding the developing oocytes (Nagahama and Yamashita 2008). The pituitary produces two types of gonadotropin, follicle-stimulating hormone (FSH) and luteinizing hormone (LH) (Brooks et al 1997). FSH acts on follicular granulosa and thecal cells, stimulating the synthesis of estradiol-17β (Suzuki et al 1998), which among other stimulates the production of a glycophosphate lipoprotein (vitellogenin) by the liver (Lubzens et al 2010; Li and Zhang 2017; Reading et al 2018). The role of LH primarily relates to the final maturation of the follicle, stimulating the production of maturation-inducing hormone (MIH) and maturation-promoting factor (MPF), leading to ovulation (Nagahama and Yamashita 2008)

Objectives

Methods

Results

Conclusion