Type collagen scaffold functionalized with ciprofloxacin loaded gelatin microspheres ndash fabrication In vitro amp In vivo evaluation histological and biochemical analysis nbsp

Abstract

The regeneration of dermis and epidermis in the soft tissue is still challenging in an infected dermal wound healing The protein based biomaterial scaffold acts as a template for an extracellular matrix in the skin Collagen biomaterials enhance healing of well infected and collagen fibres maturation by providing a scaffold for the more rapid transition to mature and aligned collagen fibres production at the wound site Gelatin denatured collagen bears no antigenicity and immunogenicity and can be easily exploited for controlled antimicrobial drug release owing to its degradation by bacterial gelatinase and collagenase present in the infected wound A porous collagen scaffold impregnated with ciprofloxacin loaded gelatin microspheres capable of delivering the drug in initially burst release followed by a controlled manner at the wound site has been developed Ciprofloxacin loaded gelatin microspheres were prepared by water in oil emulsion technique and subsequently impregnated in a collagen scaffold Collagen scaffold contained pores in the size range of micro m that encapsulate gelatin microspheres The degradation of gelatin microspheres at the wound site offers drug release at wound surface and collagen scaffold acts as a reservoir for gelatin microspheres and supports skin regeneration Morphological features of microspheres and microsphere impregnated collagen were analyzed through scanning electron microscopy SEM The SEM micrograph reveals the presence of interconnectivity pores in the scaffold and heterogenous cross sectional structures promoting cell fate process at the site of injury The encapsulation efficiency of the drug in gelatin microspheres is Drug release profile shows that of drug burst released within hours followed by controlled release up to days In vivo studies confirm gelatin microspheres impregnated in collagen scaffold heals full thickness wound created in Albino Wister rats in days whereas antibiotic incorporated collagen sponge and plain collagen scaffold heal full thickness wounds in and days respectively Histological investigation on granulated tissue concludes microspheres incorporated collagen scaffold regenerated the dermis and the epidermis effectively and controls the infection at wound site by observing fewer neutrophils in the th and th day of wound healing Masson rsquo s Trichrome staining reveals the formation of well stretched collagen bundles in the granulated tissue from treated groups and additionally the aldehyde content in the collagen of treated groups increased with the days of wound healing confirming the formation of good crosslinking in the newly synthesized collagen in the regenerated wound The biochemical analysis of granulated tissue from both open and treated wound confirms the microspheres collagen scaffold highly synthesized extracellular components in the tissue concluding microspheres impregnated collagen scaffold would be a synergistic function of regenerating the soft tissue and controlling the infection effectively results as effective wound care system