一株解烃菌TY12的分离及其特性研究

Abstract

本文以南充市炼油厂附近被石油污染获取的土壤作为研究材料，筛选分离获得一株对石油烃有降解性的疏水性烷烃降解菌TY12。经过16S rDNA的克隆测序和系统发育分析，初步确定该菌株为变形斑沙雷氏菌(Serratia proteamaculans)，并从形态学、生理生化特性对发现的菌株TY12进行了进一步的研究和分析。同时用微生物粘着碳烃化合物法(MATH)研究得到TY12菌株的表面疏水性为52.2%，且表面疏水性会随着培养时间的增加，不断的减小。该菌能在以正十二烷为唯一碳源的无机盐培养基中生长，当在含有1%(W/V)正十二烷的无机盐培养基中接入1%种子液，28℃、摇床转速180 r/min的条件下震荡培养4 d，正十二烷降解率可达66.1%。其研究成果为石油污染土壤的生物修复提供了一定的依据。 A strain of hydrophobic hydrocarbon degrading bacteria TY12 was screened out of petroleum contaminated soils from Nanchong oil refinery. Through 16S rDNA basic sequence detection and phylogenetic analysis, TY12 was identified as Serratia proteamaculans, and then according to morphological, physiology and biochemistry characteristics, this paper has a deep study on TY12. This paper studied on the cell-surface hydrophobicity of TY12 by microorganism adhering to hy-drocarbon (MATH), the results showed that the cell-surface hydrophobicity of TY12 was 52.2%, and the cell-surface hydrophobicity changed with the variation of incubation time. The n-dodecane degradation was 66.1% after TY12 was growing on in hydrocarbon degradation medium with 1% (W/V) of n-docosane at 28˚C and 180 r/min for 4 d. It had potential in bioremediation of oil con-taminated environment.