Abstract
Abstract With advances in high-sensitivity fluorescence microscopy, fluorescence correlation spectroscopy (FCS) and related fluctuation spectroscopy methods have become important and practical research tools that allow researchers to investigate the chemical and physical properties of biomolecules in a variety of complex environments (Bacia & Schwille, 2003; Chen et al., 2001; Hess et al., 2002; Krichevsky & Bonnet, 2002; Muller et al., 2003; Rigler & Elson, 2001; Schwille, 2001; Thompson et al., 2002; Webb, 2001). Specifically, fluctuation spectroscopy measurements can provide quantitative information about mobility, concentration, interactions, chemical kinetics, and physical dynamics of biomolecules both in vitro and within living cells and tissues, and the various FCS methods are being increasingly applied in many new biomedical research areas. This chapter provides an introduction to FCS, focusing primarily on the basic theoretical and experimental considerations involved in FCS measurements and analysis. Several different classes of FCS applications are also introduced to provide an overview of some key FCS capabilities.
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