Abstract
A two-frequency heterodyne confocal laser scanning fluorescence microscope (TF-CLSFM) is setup where a harmonic intensity modulated fluorescence signal is detected coherently. Due to the ability of reduction on refractive index mismatched spherical aberration in TF-CLSFM, it clearly shows that the performance of TF-CLSFM on axial response is better than conventional confocal laser scanning fluorescence microscope (CLSFM) according to the experimental results.
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