Abstract
AbstractA two‐dimensional crossed immunoelectrophoretic method for the determination of the specificity of antibodies raised against rat serum lipoproteins and apolipoproteins (Apo) is described. The method may also be used for the detection of as yet undesignated apoproteins, associated with the serum lipoprotein fractions, and exploits the superior resolving capacity of 7m urea 10% polyacrylamide gel electrophoresis for the first dimension separation and minimizes physical separation of polyacrylamide and agarose in the second dimension electrophoresis by use of low electroendosmotic flow agarose. The use of polyacrylamide instead of cellulose acetate in the first dimension permits the analysis of larger amounts of antigen protein, crossed imunoelectrophoresis of Apo E which binds to cellulose acetate, and the crossed immunoelectrophoresis of antigens previously focused to their isoelectric points in polyacrylamide gels containing ampholytes.
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