Abstract
Objectives. To investigate the stability of free and total prostate-specific antigen (PSA) in serum stored at −20°C and −70°C for 2 years. Methods. Fresh blood from 29 patients was drawn and allowed to clot at room temperature. Serum was prepared by centrifugation 1 hour later and tested to determine the free and total PSA levels. Samples were aliquoted, frozen at −20°C or −70°C, and monitored for long-term stability over the course of 2 years. Results. On average, 91% of the free PSA, 92% of the total PSA, and 89% of the percent free PSA values were recovered after 2 years of storage at −20°C; and 96% of the free PSA, 101% of the total PSA, and 95% of the percent free PSA values were recovered after 2 years of storage at −70°C. Seventy percent of the samples stored at −20°C and 100% of the samples stored at −70°C showed no statistically significant decline in free PSA values over the 2-year period. Only one total PSA sample (−20°C) showed a statistically significant loss after 2 years of storage. Conclusions. Our results suggest that samples stored frozen for up to 2 years at −70°C should give acceptable percent free PSA results.
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