Two variants of NOD2 gene polymorphisms and their relation with Tuberculosis in Andhra Pradesh population, India

Abstract

IntroductionTuberculosis (TB) remains one of the major infectious diseases even after the emergence of new diseases in the present era. Few polymorphic gene studies on Nucleotide-binding Oligomerization Domain 2 (NOD2) gene reveal that they are associated with the risk of TB in some populations. An attempt has been made to find such association in the Andhra Population of India. MethodsTwo variants (rs2066847 and rs2066842) of NOD2 gene were analyzed genotypically using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR–RFLP) analysis, on TB patients and negative controls. Statistical tests like Chi-square, Odds ratio (OR) and 95 % Confidence Interval (CI) were considered to evaluate the susceptibility of the NOD2 gene polymorphism with TB. ResultsThe analysis of the results obtained revealed that the two variants of NOD2 gene rs2066842 (OR = 1.278; 95%CI = 0.436–3.746; P = 0.653), and rs2066847 (OR = 0.809; 95%CI = 0.015–41.227; P = 0.915) do not attribute to the risk of TB in this population. ConclusionThe final results of the study reveal that these two polymorphisms of the NOD2 gene do not show an association with the susceptibility of TB in the Andhra Population of India. Further studies in different ethnic groups with larger sample sizes are necessary to evaluate the proper associative gene expression of NOD2 with TB.