Abstract
By clone purification of avian myelocytomatosis virus MC29, a virus strain, MCV-B, was isolated which was found to belong to subgroup B of avian leukosis-sarcoma viruses. It contained a nontransforming virus in excess of a transforming virus defective for reproduction. MCV-B retained the capacity to transform hematopoietic chicken tissues at a high efficiency. It was also capable of inducing two types of foci and agar suspension colonies in cell cultures derived from 11-day-old chicken embryos. These alterations were shown to be due to a morphological transformation of two different types of target cells designated s and l. Several lines of evidence indicate that a single agent is able to transform both types of target cells. Pure cultures of s-type cells were obtained by cloning of uninfected chicken cells. They were highly susceptible to transformation with Rous sarcoma virus (RSV) but resistant to transformation with avian myeloblastosis virus (AMV) and myeloblastosis associated virus (MAV). Efforts to clone-purify uninfected l-type cells have failed so far. It is suggested that this type of cell is derived from the hematopoietic system and is the in vitro target cell for transformation with AMV.
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