Abstract
Expansion and contraction instabilities associated with CAG, CGG, GAA and CGA (GAC) repeats propagation cause more than a dozen human genetic diseases and cancers. In this work, the propagation behavior of a bacteriophage M13 carrying a calf prochymosin cDNA fragment with a (CGA) 2 repeat in a small hairpin forming region is reported. Such a M13 derivative when propagated in Escherichia coli, produces small plaques by decreasing phage yield and also mitigates the inhibition on host cell growth, compared to those control bacteriophages either containing a “CTGCTA” sequence or wildtype, suggesting that CGA 2 repeat impedes DNA replication in vivo. Moreover, an increased internal free energy is found associated with (CGA) 2 sequence compared to those “CTGCTA” and wildtype, which ruled out a possibility of CGA 2 repeat effects on propagation is through influencing the hairpin structure formation.
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