Abstract
To cure Escherichia coli for plasmids derived from the ColE1 replicon advantage is taken of the fact that maintenance of this replicon requires a wild-type allele of polA, encoding DNA polymerase I. Curing is achieved by cotransduction of a mutant polA allele with metE::Tn 10, fadAB::Tn 10 or other transposon insertions near polA. Reciprocal transduction to Met + Pol + or to Fad + Pol + ensures reestablishment of the original genotype except for loss of the plasmid. A set of useful bacterial strains is provided.
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