Abstract
A two-stage semi-continuous strategy for producing 2-keto-gluconic acid (2KGA) by Pseudomonas plecoglossicida JUIM01 from rice starch hydrolyzate (RSH) has been developed. The initial glucose concentration (140 g/L) was selected for first-stage fermentation due to its highest 2KGA productivity of 7.58 g/(L⋅h), cell weight of 3.91 g/L, and residual glucose concentration of 25.00 g/L. Followed by removing 70.0% (v/v) of the first-stage broth and feeding 400.0 g/L of glucose to the second-stage fermentor, a total of 50680.0 g glucose was consumed, and 50005.20 g 2KGA was obtained with a yield of 0.9867 g/g by P. plecoglossicida JUIM01 after a 3-cycle two-stage semi-continuous fermentation. Our results indicated that the developed two-stage semi-continuous fermentation could be industrially applied due to its high 2KGA concentration, 2KGA yield and operation efficiency.
Highlights
Erythorbic acid, known as D-isoascorbic acid, is a stereoisomer of ascorbic acid
Each second-stage fermentation ended when glucose concentration was below 1.0 g/L
A two-stage semi-continuous fermentation has been developed with the cooperation of 3 fermentors to enhance the 2-Keto-D-gluconic acid (2KGA) production performance by P. plecoglossicida JUIM01 using rice starch hydrolyzate (RSH) as the substrate
Summary
Erythorbic acid, known as D-isoascorbic acid, is a stereoisomer of ascorbic acid. Erythorbic acid and its salts have seen their applications extended to include roles in mushroom post-harvesting as an oxygen scavenger, in. 2-Keto-D-gluconic acid (2KGA) is a key intermediate for erythorbic acid and erythorbate production. It is a versatile chemical applied in the cosmetics and pharmaceutical industries (Li et al, 2016). For 2KGA preparation, at least three methods including chemical synthesis, enzymatic conversion, and microbial fermentation have been proposed. Chemical and enzymatic methods had limits to large-scale production due to their critical reaction conditions, low yield and/or high process costs (Elseviers et al, 2000; Tanimura et al, 2003). Microbial fermentation is the main industrial 2KGA production method with two consecutive oxidations by glucose dehydrogenase and gluconate dehydrogenase
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