Abstract
The method of two-stage rocket electrophoresis on gelatinized cellulose acetate film (Cellogel) was developed. The method is based on electroimmunodiffusion detection of antigen on cellulose acetate films containing monospecific antiserum of a test system, followed by detection of precipitation bands with the appearance of stained “rockets” if the reaction takes place in the visible zone, or with further treatment of cellulose acetate strips containing invisible precipitates with antiglobulin antibodies, complement, or a combination of both. The sensitivity of the method is increased to 30–60 ng/ml in the visible zone of reaction by simultaneously reducing the concentration of antibodies and increasing the absolute amount of antigen for electrophoresis to 50–100 λl. Details of the method were worked out for human α-fetoprotein.
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