Abstract
Stress in microalgae through the metabolic pathway increases the value of lipids and caratenoids. In this study, two stage feeding in Chlorella pyerenoidosa and Chlorella vulgaris are evaluated. BBM for Chlorella vulgaris and Sorrokin/Krauss Chlorella pyrenoidosa are using as standar medium which is label as A0/B0, cow manure Medium with fluorescence light exposure labelled as A1/B1, with sunlight exposure labelled as A2/B2 plus urea 0.1 g/l [1] with fluorescence rays exposureas A3/B3.v Two stage feeding is evaluated in A4/B4 that compose medium A3/B3 which is added to A1/B1 medium on reaching the stationary phase. Which is Chlorella pyrenoidosa is labeled as A and Chlorella vulgaris in label B. The highest accumulation of biomass in Chlorella species was seen with the addition of urea in A3 and A4, but it had the lowest lipid content. During the experiment with the addition of water and nutrients from cow manure, the lipid values visibly increased, but not optimally. In Chlorella pyrenoidosa and Chlorella vulgaris species which is the object of this study, the lipid values on A1 and B1 are higher than the control and treatment with sunlight exposure, as well as the addition of urea.
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