Two quorum sensing enhancement methods optimized the biofilm of biofilters treating gaseous chlorobenzene

Abstract

In this study, effects of two quorum sensing (QS) enhancement methods on the performance and biofilm of biofilters treating chlorobenzene were investigated. Three biofilters were set up with BF1 as a control, BF2 added exogenous N-acyl-homoserine lactones (AHLs) and BF3 inoculated AHLs-producing bacterium identified as Acinetobacter. The average chlorobenzene elimination capacities were 73 and 77 g/m3/h for BF2 and BF3 respectively, which were significantly higher than 50 g/m3/h for BF1. The wet biomass of BF2 and BF3 with QS enhancement eventually increased to 60 and 39 kg/m3 respectively, and it was 29 kg/m3 for BF1. Analysis on biofilms in three biofilters showed that distribution uniformity, extracellular polymeric substances production, adhesive strengths, viability, and metabolic capacity of biofilms were all prompted by the two QS enhancement methods. Comparisons between the two QS enhancement methods showed that adding exogenous AHLs had more significant enhancing effect on biofilm due to its higher AHLs level in start-up period, while AHLs-producing bacteria had an advantage in enhancing bacterial community diversity. These results demonstrate that QS enhancement methods have the potential to optimize the biofilm and thus improve the performance of biofilters treating recalcitrant VOCs.