Abstract
Due to the unique advantages of two-photon technology and time-resolved imaging technology in the biomedical field, attention has been paid to them. Gold clusters possess excellent physicochemical properties and low biotoxicity, which make them greatly advantageous in biological imaging, especially for in vivo animal imaging. A gold nanocluster was coupled with dihydrolipoic acid to obtain a functionalized nanoprobe; the material displayed significant features, including a large two-photon absorption cross-section (up to 1.59 × 105 GM) and prolonged fluorescence lifetime (>300 ns). The two-photon and time-resolution techniques were used to perform cell imaging and in vivo imaging.
Highlights
The imaging of living animals is of extreme importance in the study of biotic changes and for the valuable information provided by disease diagnoses and health assessments at different levels, such as organs, tissues, etc
Magnetic resonance imaging (MRI), computed tomography (CT), and high-frequency micro-ultrasound are often used for anatomical imaging, and for molecular visualizations, optical imaging, positron emission tomography (PET), and single-photonemission computed tomography (SPECT) are used [1]
The emission spectrum was centered at 617 nm, and a full width at half maxima (FWHM) of 106 nm was observed in ddH2 O
Summary
The imaging of living animals is of extreme importance in the study of biotic changes and for the valuable information provided by disease diagnoses and health assessments at different levels, such as organs, tissues, etc. Among the many modalities of imaging, non-invasive and in vivo imaging are especially significant in studying long-term animal models. Magnetic resonance imaging (MRI), computed tomography (CT), and high-frequency micro-ultrasound are often used for anatomical imaging, and for molecular visualizations, optical imaging (including fluorescence imaging and bioluminescence imaging), positron emission tomography (PET), and single-photonemission computed tomography (SPECT) are used [1]. With the emergence of the femtosecond laser, two-photon fluorescence imaging rapidly developed. It has gained more advantages than one-photon fluorescence imaging. Because the laser beam of two-photon excitation is focused at a focal point, the potential spatial resolution is improved, and photo-bleaching is reduced [8]
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