Abstract
Synthesis and photophysical properties of coumarin–DBD FRET pairs, which are also suitable for two-photon excitation and fluorescence-lifetime imaging microscopy.
Highlights
The synthesis and photophysical properties of two new Forster Resonance Energy Transfer (FRET) pairs based on coumarin as a donor and DBD dye as an acceptor are described
A longwavelength absorption maximum is usually connected with low thermal and photochemical stability of the dyes. This problem can be circumvented by two-photon excitation (2PE),[4] which is based on the simultaneous absorption of two photons with lower energy instead of one photon with high energy
A Excited at 780 nm (2, 8, 10a,b) or 720 nm (4), GM 1⁄4 10À50 cm[4] per s per photon. b Fluorescence lifetime was detected at 420 nm. c Fluorescence lifetime was detected at 570 nm. d Fluorescence lifetime was detected at 570 nm
Summary
The synthesis and photophysical properties of two new FRET pairs based on coumarin as a donor and DBD dye as an acceptor are described. The potential of a uorescent dye is determined by the combination of its photophysical parameters, such as uorescence quantum yield (FF), molar extinction coefficient (3), uorescence lifetime (sF), absorption and emission wavelength (lABS, lEM), and stability against photobleaching. S2 is low for most organic molecules.[5] On the other hand the applicability of many uorescence dyes suitable for 2PE is limited when considering the other photophysical properties.
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