Abstract
RATIONALE: Eosinophils trigger symptoms in allergic rhinitis. The current method for diagnosis of nasal eosinophils is through nasal cytology. New diagnostic methods for identifying nasal eosinophils through autofluorescence of flavin adenine dinucleotide could offer a novel approach without the need for fixation or staining. Two-photon excitation is a powerful method for detecting this fluorescence. We aim to characterize the performance of two-photon excited fluorescence (TPEF) to detect eosinophils in this proof-of-concept study for a future miniature in vivo imaging instrument.
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