Abstract
Only 3% of phage genomes in NCBI nucleotide database represent phages that are active against Streptococcus sp. With the aim to increase general awareness of phage diversity, we isolated two bacteriophages, Str01 and Str03, active against health-threatening Group A Streptococcus (GAS). Both phages are members of the Siphoviridae, but their analysis revealed that Str01 and Str03 do not belong to any known genus. We identified their structural proteins based on LC–ESI29 MS/MS and list their basic thermal stability and physico-chemical features including optimum pH. Annotated genomic sequences of the phages are deposited in GenBank (NCBI accession numbers KY349816 and KY363359, respectively).
Highlights
Bacteria from the genus Streptococcus are well known to cause a serious healthcare burden
Isolation and basic characterization of two new temperate streptococcal phages Bacteriophages were isolated from a clinical isolate of S. pyogenes (Polish Collection of Microorganisms at the Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences (PCM) (PCM accession no. 2855), from plaques on agar plates after spontaneous release
The phage host range was tested on a set of 37 clinical isolates of Group A Streptococcus (GAS) and 34 clinical isolates of Group B Streptococcus (GBS) (S3 Table)
Summary
Bacteria from the genus Streptococcus are well known to cause a serious healthcare burden. Isolated phages were named Str01 and Str03, and were deposited in PCM (accession numbers 595-PH and 597-PH, respectively) and used for genomic, morphological and stability studies. We used lysogens from the host strain and used this as positive control (bacteria with phage-specific DNA).
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