Two novel species of Neoaquastroma (Parabambusicolaceae, Pleosporales) with their phoma-like asexual morphs.

Chayanard Phukhamsakda,Marc Stadler,Sinang Hongsanan,Jian-Chu Xu,Darbhe J Bhat,Kevin D Hyde

doi:10.3897/mycokeys.34.25124

Chayanard Phukhamsakda, Marc Stadler + Show 4 more

Open Access

https://doi.org/10.3897/mycokeys.34.25124

Copy DOI

Abstract

The monotypic genus Neoaquastroma (Parabambusicolaceae, Pleosporales) was introduced for a microfungus isolated from a collection of dried stems of a dicotyledonous plant in Thailand. In this paper, we introduce two novel species, N. bauhiniae and N. krabiense, in this genus. Their asexual morphs comprise conidiomata with aseptate and hyaline conidia. Neoaquastroma bauhiniae has ascomata, asci and ascospores that are smaller than those of N. krabiense. Descriptions and illustrations of N. bauhiniae and N. krabiense are provided and the two species compared with the type species of the genus, N. guttulatum. Evidence for the introduction of the new taxa is also provided from phylogenetic analysis of a combined dataset of partial LSU, SSU, ITS and tef1 sequence data. The phylogenetic analysis revealed a distinct lineage for N. bauhiniae and N. krabiense within the family Parabambusicolaceae.

Highlights

Thailand is a highly biodiverse country in the tropics with hot and humid climate (MacKinnon et al 1986, Marod and Kutintara 2012)
Pure cultures were obtained from single ascospores on malt extract agar (MEA; 62 g/l) in distilled water following the method of Chomnunti et al (2014)
We introduce two new species of Neoaquastroma, as N. bauhiniae and N. krabiense

Summary

Introduction

Thailand is a highly biodiverse country in the tropics with hot and humid climate (MacKinnon et al 1986, Marod and Kutintara 2012). The family Parabambusicolaceae was introduced for a distinct phylogenetic lineage in the suborder Massarineae (Pleosporales) (Tanaka et al 2015). Species of Parabambusicolaceae are characterised by pseudothecioid ascomata with or without stromatic tissues, papillate to apapillate ostioles, clavate to fusiform asci and hyaline or brown phragmospores (Liu et al 2015, Tanaka et al 2015, Li et al 2016, Wanasinghe et al 2017). The genus Neoaquastroma Wanas., E.B.G. Jones & K.D. Hyde, has been introduced from a dead twig of a herbaceous plant collected in Northern Thailand and been typifed with N. guttulatum Wanas., E.B.G. Jones & K.D. Hyde (Wanasinghe et al 2017). The genus is characterised by immersed, glabrous pseudothecia, short, papillate, fissitunicate, clavate asci and ellipsoidal to subfusiform, multi-septate hyaline phragmospores, surrounded by a mucilaginous sheath (Wanasinghe et al 2017). Molecular phylogenetic analysis using ribosomal DNA (LSU, SSU and ITS) and translation elongation factor 1-alpha (tef1) sequence data support it as a distinct genus in Parabambusicolaceae

Objectives

Methods

Results

Conclusion