Abstract
Sodium channels play a critical role in the generation and propagation of action potentials in excitable tissues, such as nerves, cardiac muscle, and skeletal muscle, and are the primary targets of toxins found in animal venoms. Here, two novel peptide toxins (Cl6a and Cl6b) were isolated from the venom of the spider Cyriopagopus longipes and characterized. Cl6a and Cl6b were shown to be inhibitors of tetrodotoxin-sensitive (TTX-S), but not TTX-resistant, sodium channels. Among the TTX-S channels investigated, Cl6a and Cl6b showed the highest degree of inhibition against NaV1.7 (half-maximal inhibitory concentration (IC50) of 11.0 ± 2.5 nM and 18.8 ± 2.4 nM, respectively) in an irreversible manner that does not alter channel activation, inactivation, or repriming kinetics. Moreover, analysis of NaV1.7/NaV1.8 chimeric channels revealed that Cl6b is a site 4 neurotoxin. Site-directed mutagenesis analysis indicated that D816, V817, and E818 observably affected the efficacy of the Cl6b-NaV1.7 interaction, suggesting that these residues might directly affect the interaction of NaV1.7 with Cl6b. Taken together, these two novel peptide toxins act as potent and sustained NaV1.7 blockers and may have potential in the pharmacological study of sodium channels.
Highlights
Voltage-gated sodium channels are essential for the initiation and propagation of action potentials that generate electrical impulses in neurons, cardiac muscle, and skeletal muscle, as well as in other excitable cells [1]
Sodium channel α-subunits are composed of four homologous domains (DI–IV), each containing six hydrophobic transmembrane segments (S1–S6) [1,2]
The S5–S6 helices of each α-subunit domain contribute to pore channel formation, which is responsible for Na+ conduction across the membrane
Summary
Voltage-gated sodium channels are essential for the initiation and propagation of action potentials that generate electrical impulses in neurons, cardiac muscle, and skeletal muscle, as well as in other excitable cells [1]. Sodium channels are composed of a pore-forming α-subunit and one or two auxiliary β-subunits [1,2]. Four sodium channel β-subunits (β1–β4) have been identified in mammals. Sodium channel α-subunits are composed of four homologous domains (DI–IV), each containing six hydrophobic transmembrane segments (S1–S6) [1,2]. The S5–S6 helices of each α-subunit domain contribute to pore channel formation, which is responsible for Na+ conduction across the membrane. The S1–S4 helices of each domain constitute the “voltage sensor”, which detects changes in membrane potential.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
