Abstract
Neptunea arthritica cumingii (Nac) is a marine snail with high nutritional and commercial value; however, little is known about its active peptides. In this study, two multi-functional peptides, YSQLENEFDR (Tyr-Ser-Gln-Leu-Glu-Asn-Glu-Phe-Asp-Arg) and YIAEDAER (Tyr-Ile-Ala-Glu-Asp-Ala-Glu-Arg), were isolated and purified from meat and visceral mass extracts of Nac using a multi-bioassay-guided method and were characterized by using liquid chromatography-tandem mass spectrometry. Both peptides showed high antioxidant, angiotensin-converting enzyme (ACE)-inhibitory, and anti-diabetic activities, with half-maximal effective concentrations values less than 1 mM. Antioxidant and ACE-inhibitory activities were significantly higher for YSQLENEFDR than for YIAEDAER. In a zebrafish model, the two peptides exhibited strong scavenging ability for reactive oxygen species and effectively protected skin cells against oxidative damage without toxicity. Molecular docking simulation further predicted the interactions of the two peptides and ACE. Stability analysis study indicated that the two synthetic peptides maintained their activities under thermal stress and simulated gastrointestinal digestion conditions. The low molecular weight, high proportion of hydrophobic and negatively-charged amino acids, and specific C-terminal and N-terminal amino acids may contribute to the observed bio-activities of these two peptides with potential application for the prevention of chronic noncommunicable diseases.
Highlights
Marine taxa are rich in bioactive compounds [1] that show antioxidative, antihypertensive, anti-diabetic, antimicrobial, and antitumor bioactivities [2], and are potentially valuable for the prevention and treatment of chronic noncommunicable diseases (NCDs) [3,4]
The proximate compositions of the meat and visceral mass are summarized in Figure S1 of the Supplementary Materials
MTZ or peptides), more fluorescent spots (FS) were detected for M-P6 at high concentrations (Figure 4b(F)). The reason for this result may be superior reactive oxygen species (ROS) scavenging ability of M-P6 promoting more skin cells regeneration. These results indicated that the two peptides could protect the skin cells of transgenic zebrafish against oxidative damage caused by MTZ
Summary
Marine taxa are rich in bioactive compounds [1] that show antioxidative, antihypertensive, anti-diabetic, antimicrobial, and antitumor bioactivities [2], and are potentially valuable for the prevention and treatment of chronic noncommunicable diseases (NCDs) [3,4]. Mollusks are the second largest phylum of the animal kingdom In addition to their ecological roles, they have great commercial value as food [6]. Little is known about these active compounds, and mollusks are a relatively undeveloped resource for high-value products. The antioxidant, ACE-inhibitory, and anti-diabetic activities were evaluated in vitro, and the bioactivity and toxicity were evaluated in vivo in a zebrafish model (Danio rerio). Owing to their rapid organogenesis, transparent embryos, and high genetic similarity with humans [18], zebrafish are used extensively for studies of human diseases and activity screening [19]. To examine the therapeutic feasibility, the stabilities of the synthetic peptides for maintaining 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH) radical scavenging activity and ACE-inhibitory activity under thermal stress and exposure to gastrointestinal digestion were investigated
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
